| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| atoh7 | xenopus |
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Experiment details for atoh7
The ETS transcription factor Etv1 mediates FGF signaling to initiate proneural gene expression during Xenopus laevis retinal...The ETS transcription factor Etv1 mediates FGF signaling to initiate proneural gene expression during Xenopus laevis retinal development.
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| Fig. 2. Loss of Etv1 function prevents proneural gene expression. Etv1 function was blocked by injection of etv1 MO at the 8-cell stage (B,E,H,K,N,Q,T) or by transgenic expression of dnEtv1 using the eye-specific enhancer for human fzd5 (C,F,I,L,O,R,U). In situ hybridization on stage 28 embryos showed that expression of early retinal markers were unaffected relative to the uninjected side, including rax (A and B, n = 78/82 unaffected; C, n = 32/32), pax6 (D and E, n = 59/65; F, n = 15/15), sox2 (G and H, n = 51/58; I, n = 28/28), and the pre-neurogenesis bHLH gene ascl3 (J and K n = 37/37; L, n = 32/32). Expression of proneural bHLH genes required for retinal neurogenesis was reduced compared to the uninjected side: atoh7 (M and N, n = 70/124 reduced; O, n = 56/98), neurog2 (P and Q, n = 39/61; R, n = 20/34), and neurod1 (S and T, n = 53/103; U, n = 18/28). Arrows indicate the eye. | |||||||||||
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| Fig. 5. Etv1 overexpression does not trigger early retinal neurogenesis. Early retinal patterning genes rax (A–C) and pax6 (D–F) were unaffected by 8-cell injection of mRNA for etv1. The proneural bHLH gene atoh7 (G, n = 49) and neurod1 (J, n = 76) were not expressed prematurely at stage22/23, indicating that etv1 was insufficient to initiate their expression. At stage 28, expression of both atoh7 (H and I) and neurod1 (K and L) was reduced on the injected side compared to the uninjected side (n = 24/48 for atoh7; n = 20/34 for neurod1). Arrows indicate the eye. |