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Figure 2.
Yap knockdown decreases eye size and EdU incorporation in the post-embryonic retina.
(A) Lateral views (left panels), head dorsal views (middle panels) and dissected eyes (right panels) of stage 40 tadpoles following two-cell stage microinjection of Yap-5-mismatch-MO (control) or Yap-MO. The asterisk indicates the injected side. (B) Quantification of dissected eye area. (C) Eyes of stage 40 tadpoles following optic vesicle transplantation at stage 25 as shown in the schematic. Dotted lines delineate the eye circumference. (D) EdU incorporation assay (6-hr pulse) analyzed on retinal sections from stage 40 tadpoles injected as in (A). Higher magnifications of the CMZ (delineated by dotted lines) are shown for each condition. (E) Quantification of EdU-positive cells within the whole CMZ or within the most peripheral stem cell compartment. (F) In situ hybridization analysis of Hes4 (retinal stem cell marker; El Yakoubi et al., 2012) and Atoh7 (progenitor cell marker; Kanekar et al., 1997) expression on stage 40 retinal sections from tadpoles injected as in (A). (G–J) Hoechst staining and PCNA immunolabeling on stage 40 retinal sections from tadpoles injected as in (A). The CMZ is delineated with dotted lines. The number of analyzed retinas per condition is indicated in each bar. Data are represented as mean ± SEM. Scale bar = 1 mm in (A) and 40 µm in other panels.
DOI: http://dx.doi.org/10.7554/eLife.08488.007 |
