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Fig. 6. Vxn is required for retinal neuron differentiation. (A–C) mRNA for GFP (300 pg) was injected alone or together with 3 ng of vxn mismatch MO-A or vxn MO-A into 1 dorsal blastomere at the 32-cell stage. Embryos were cryosectioned at stage 41, and GFP-labeled retinal cell types were counted. vxn MO-A caused a significant decrease in all retinal neuron types, and a significant increase in Muller glia (MG) and/or neuroepithelial cells (NEP) (A). A section of stage 41 retina showing many GFP-labeled retinal cells with the morphology of Muller glia and/or NEPs (B). Immunostaining for CRALBP to distinguish MG from NEPs showed that both populations increased significantly (C). (D–I) Injection of 20 ng of vxn MO-B at 8CS did not alter atoh7 expression in stage 34–35 embryos (D, E), but prevented expression of hermes (F, G) and barhl2 (H, I) on the injected (inj) side (brackets; E, G,I) when compared to the uninjected (uninj) side (D, F, H). RGC, retinal ganglion cells; HC, horizontal cells; AC, amacrine cells; BP, bipolar cells; PR, photoreceptor cells; MG, Muller glial cells; NEP, neuroepithelial cells. *p < 0.001 and #p < 0.01 by Student's t-test. |
