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Experiment details for atoh7

Willardsen MI et al. (2009) Assay

Temporal regulation of Ath5 gene expression during eye development.

Gene Clone Species Stages Anatomy
atoh7.S laevis NF stage 28 retina , eye , optic vesicle

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  Fig. 6. Endogenous Xath5 expression is regulated by FGF signaling. Stage 28 Fzd5-XFD transgenic embryos show decreased expression of bHLH transcription factors Xath5 (B), NeuroD (D) vs. non-transgenic controls (A, C). The expression of retinal progenitor genes Rx (E–F), and Sox2 (G–H) is not affected. Retinal expression of Xer81 is inhibited in Fz5-XFD transgenic embryos (J) vs. non-transgenic controls (I).

Gene Clone Species Stages Anatomy
atoh7.S laevis NF stage 31 retina , eye , optic vesicle

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  Fig. 4. Pax6 is required for Xath5 expression but is not sufficient to induce Xath5 expression. Overexpression of dnPax6 mRNA decreases Xath5 expression on the injected side (A–B) but does not affect expression of the progenitor markers Vsx1 (C–D) or Rx (E–F). Overexpression of Pax6 mRNA is not sufficient to induce ectopic Xath5 expression (G–H) or ectopic pG1X5 3.3 kb transgene expression (I–J). Gut autofluorescence.