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bcanxenopus regenerating tail 

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Experiment details for bcan

Unique Gene Expression Profile of the Proliferating Xenopus Tadpole Tail Blastema Cells Deciphered by RNA-Sequencing Analysis.

Unique gene expression profile of the proliferating Xenopus tadpole tail blastema cells deciphered by RNA-sequencing analysis.

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Gene Clone Species Stages Anatomy
bcan.L laevis NF stage 49 to NF stage 53 regenerating tail

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  Fig 5. Expression analysis of candidate genes in regenerating tadpole tails by whole mount in situ hybridization.(A-E) Whole mount in situ hybridization using 3-dpa regenerating tails of St. 49-53 tadpoles for (A) interleukin 11, (B) keratin 18, (C) brevican, (D) cse1l, and (E) lysyl oxidase (blue/purple). (F-M) Sagittal sections of tail blastema from 3-dpa regenerating tails of St. 49-53 tadpoles double stained by whole mount in situ hybridization for (F-I) interleukin 11 and (J-M) keratin 18 (magenta) and BrdU-immunohistochemistry (green). (G) and (K) show magenta channels (mRNA), (H) and (L) show green channels (BrdU), (I) and (M) show blue channels (nuclei stained with Hoechst 33342), and (F) and (J) show merged images. Note that mRNA signals detected in the cytoplasm do not exactly merge with BrdU-signals detected in the nuclei. White broken lines indicate cell populations that highly expressed the genes. Anterior is to the left, and dorsal is up. Scale bars indicate 500 μm in (A-E) and 100 μm in (F-M).