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Figure 4. Spatial expression patterns of embryonic and epidermal RNA splicing isoforms. (A) Representations of the variety of splicing isoforms expressed in the ACs or in whole embryo for the indicated genes. (B) RNA in situ hybridizations for age-matched embryos and ACs at indicated stages, hybridized either with the skin specific splicing isoform probes (CPNE1 RNA0071, BMP7.2 RNA13257 and EVPL X1) or with the embryo-specific isoform probes (CPNE1 RNA0068, BMP7.2 RNA13254). Genes were picked from Supplementary Table S7; bmp7.2 is not on the list, since ACs and embryo share the same prevalent isoform. It was nevertheless chosen because of the importance of BMP signaling in ectodermal cell fate choice. Inserts show embryos hemisectioned prior to hybridization. Staining reactions have been conducted in parallel for each row. |
