Too many results?Too few results?
Experiment details for btbd3Measuring Absolute RNA Copy Numbers at High Temporal Resolution Reveals Transcriptome Kinetics in Development.
Measuring Absolute RNA Copy Numbers at High Temporal Resolution Reveals Transcriptome Kinetics in Development.
Display additional annotations [+]
|Isoform Differential Expression in Clutch A, see also Fig S5 A – Isoform switching event examples: maternal to maternal; maternal to zygotic; and zygotic to zygotic switching events (left). Histogram of isoform switching events by category and time (right). B – bicd2 isoforms switch in PolyA+ data (circles), and do not switch in rdRNA data (squares) indicating differential polyadenylation. PolyA+ and rdRNA abundances agree within uncertainty of the absolute normalization (Supplemental Experimental Procedures). C – Normalized read depth on bicd2 locus in Clutch A polyA+ between 0–4.5 hpf. Note temporal dynamics shared by the final three bicd2(1) exons. Heatmap and depth of pile ups corrected for changing total mRNA. D – Spatial expression of three genes with isoforms with differential dynamics. All isoforms show different temporal and spatial expression domains. Grey lines and points mark total expression for each gene (sum of the red and blue isoforms).|