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Figure 4. Midbrain histological analysis of voltage-gated calcium channel (VGCC) alpha 1 subunit expression in stages 35-37 (swimming tadpole) X. laevis using whole-mount in situ hybridization. A-J: Transverse 18- mu m sections within the midbrain region of the anterior-posterior axis, with dorsal to the top. A,B: CaV1.2 expression. C: CaV1.3 expression. D,E: CaV2.1 expression. F,G: CaV2.2 expression. H,I: CaV3.1 expression. J: CaV3.2 expression. Arrowheads indicate regions of expression. mb, midbrain; e, eye; ht, heart; V, cranial ganglion V, trigeminal ganglion; VII, cranial ganglion VII, geniculate (facialis) ganglion. Scale bar = 0.25 mm. |