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Fig. 5. XCD44 gene expression pattern. (A) XCD44 expression during Xenopus development analyzed by RT-PCR. (B) Dorsal view of a stage 20 embryo showing XCD44 mRNA localization in the presomitic mesoderm. (C) Lateral view of a stage 24 embryo: XCD44 gene expression is detected in somites, cement gland (arrowhead) and, at lower level, in the cranial NCC (arrows). (C') Bisected embryo at stage 24 showing that in the trunk region XCD44 is localized exclusively in the somites. The notochord (n) at this stage does not express XCD44 mRNA. (D) Lateral view of stage 32 embryo: XCD44 mRNA is present in somites, branchial arches (arrow) and in the otic vesicle (arrowhead). A horizontal section at the level of the otic region is shown in the inset. (E) Lateral view of a stage 37 embryo showing the localization of XCD44 transcripts in the CNS (arrow), the dorsal somite tips (black arrowhead), the notochord and migrating hypaxial muscle cells (red arrowheads). (F-H) Dorsal view of XCD44-Mo injected embryos at neurula stage, the injected side of the embryos is visualized by the Red-gal staining. The expression of the following markers was detected by whole mount in situ hybridization: (F) MyoD, (G) p27 and (H) CA. (I) An example of a XCD44-Mo injected embryo processed by TUNEL assay. The apoptotic cells are in blue; red spots indicate the site of injection. |
