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Fig. S8. PKA stimulation and CREB overexpression enhance P-CREB levels in the developing spinal cord. (A) CREB activation is apparent in the developing
spinal cord. Neural plate and spinal cord transverse sections from wild-type embryos were processed for P-CREB (green) and Sox2 (red, neural progenitor
marker) immunostaining. Shown are representative examples of transverse immunostained sections of the neural plate (Top) and spinal cord (Bottom)
(outlined). (Scale bar: 20 μm.) (B) PKA activates CREB in the embryonic spinal cord. Western blot assays from spinal cord homogenates probed with antibodies
for P-CREB and H2B (loading control). Samples were incubated in the absence or presence of 20 μM forskolin for 40 min. Shown is a representative example.
(C) Expressing CREB induces enhanced levels of P-CREB in the neural plate. Two-cell-stage embryos were unilaterally injected with CREB-flag mRNA, grown
until neural plate stages, and then were processed for P-CREB (green), flag (red), and Sox2 (blue) immunostaining. Shown is a representative example of
a transverse immunostained section of the neural plate (outlined). (Scale bar: 30 μm.) |
