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Figure 6
Oocytes exclusively express CYP17, whereas follicular cells contain all the 3β-HSD and 17β-HSD activity in Xenopusovaries. Pregnenolone, progesterone DHEA, and AD metabolism were examined in isolated oocytes (A), follicular cells (B), or recombined follicular cells and oocytes (C), by incubating cells with the respective radiolabeled steroids at 100 nM for the indicated times. Steroids were then extracted from the medium and examined by TLC. Locations of steroids are indicated. All experiments were performed at least three times using similar numbers of cells with nearly identical results. |
