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Fig. 2. Expression of X-bhh, X-chh and X-shh on retinal sections. These panels show retinal vibratome sections after whole-mount in situ hybridisation. The probes are indicated for each panel. (A,C,E) Retinas of stages 34-36 embryos. (B,D,F) Retinas of stage 42 embryos. (A) X-shh is expressed in the RPE (arrow) and in the ganglion cell layer (arrowheads). (B) X-shh is strongly expressed in ganglion cells (arrowheads) and a faint staining is visible in a cluster of cells in the outer layer and the RPE (arrow). (C,E) X-bhh and X-chh are expressed in the RPE (arrows). (D,F) X-bhh and X-chh are still strongly expressed in the RPE (arrows), excluding the peripheral pigmented epithelium (bars). le, lens. Dorsal is on the top. Scale bar: 40 μm. |
