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Figure 1—figure supplement 1. Characterization of Sox17-MO embryos and Sox17-regulated transcriptome.
(A) Temporal expression of key endoderm GRN transcripts (GSE65785; Owens et al., 2016). (B) Sequence alignment of antisense sox17a-MO and sox17b-MO (red) with 5’ end of Xenopus tropicalis sox17a, sox17b.1 and sox17b.2 mRNAs (black) spanning the ATG translation start (green). (C) In situ hybridization of markers for the liver (nr1h5), stomach, lung, intestine and pharynx (osr1 and islet1) snowing that co-injection of mouse Sox17 RNA rescues gut development in sox17-MO embryos at the tailbud stage. (D) Additional validation of gastrula stage transcripts downregulated (otx2, admp, dhh and gata4) or upregulated (wnt8 and nodal1) in sox17-MO embryos and rescue by co-injection of mSox17. (E) GO term enrichment analysis of Sox17-regulated genes showing fold enrichment by Fisher’s exact test with p-values adjusted to 5% FDR for multiple testing correction. |
