| |
Fig. 1. Schematic representation of the RNA-seq experiment. A) The early organizer from stage 10+ embryos and the dorsal and the ventral marginal zone (DMZ and VMZ) explants from mid-gastrula stage embryos were dissected and subjected to RNA sequencing. Cells in these regions have distinct fates and motile behaviors. Pairwise comparison of differential expressed genes was performed between the organizer and the DMZ, and the DMZ and the VMZ, samples. The ANOVA-like analysis for all three samples sets was also performed, which produced similar results as that in the pairwise comparison. Genes with potential to regulate embryonic patterning and/or movements were especially scrutinized in more detail in this study. B) A list of the selected known and uncharacterized genes with differential expression patterns in different embryonic regions is shown. |
