|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
isl1
|
|
tropicalis
|
NF stage 35 and 36
|
midbrain
,
spinal cord
,
stomach
,
intestine
,
eye
,
[+]
|
osr1
|
|
tropicalis
|
NF stage 35 and 36
|
cement gland
,
lung
,
stomach
|
admp
|
|
tropicalis
|
NF stage 10.5
|
involuted dorsal mesoderm
,
endoderm
,
axial mesoderm
,
dorsal endomesoderm
|
nr1h5
|
|
tropicalis
|
NF stage 35 and 36
|
liver
|
wnt8a
|
|
tropicalis
|
NF stage 10.5
|
ventro-lateral marginal zone
,
involuted ventral mesoderm
|
ventx3
|
|
tropicalis
|
NF stage 10.5
|
ventro-lateral marginal zone
,
ventral endoderm
|
|
|
Figure 3—figure supplement 1. Validation of Bcat-MO embryos.
(A) Immunostaining of Tg(WntREs:dEGFP)Vlem Xenopus tropicalis gastrula embryos show the loss of nuclear Bcat and GFP expression from the Wnt-reporter transgene in Bcat-MO embryos demonstrate effective knockdown. (B–C) Co-injection of RNA encoding a human S37A-stabilized Bcat recues the Bcat-MO-ventralized phenotype. In situ hybridization of gastrula (B) and tailbud embryos (C). In Bcat-MO gastrula, fst and admp are downregulated in the dorsal organizer, whereas wnt8a and ventx3.1 are upregulated consistent with a ventralized phenotype. (C) In situ hybridization of markers for the liver (nr1h5), stomach, lung, intestine and pharynx (osr1 and islet1) show that co-injection of human S37A-stabilized Bcat RNA rescues the ventralized phenotype and disrupted gut development in Bcat-MO tailbud embryos. |