||Fig. 4. Whole-mount in situ hybridization of XGAP-43 during Xenopus laevis embryogenesis. Numbers in the left column represent the approximate stage
of embryos in each row. Embryos in columns (A), (B), (D) and (E) were incubated with a full-length antisense XGAP-43 probe and those in columns (C)
and (F) with full-length sense XGAP-43. Lateral views of XGAP-43 expression are shown in columns (A) and (D-F), and dorsal views in column (B).
XGAP-43 transcripts were first observed around stage 22 where they appeared as two parallel stripes on either side of the midline, and also in bilateral
regions of the prospective forebrain/midbrain border. In later stages, XGAP-43 expression was observed in a complex spatiotemporal pattern throughout
the nervous system. E: magnification of CNS structures expressing XGAP-43 in a stage 34 embryo: 1 = brainstem; 2 = hindbrain; 3- cerebellum;
4 = midbrain; 5 = pineal gland; 6 = forebrain (olfactory region); 7 = diencephalon; 8 = optic vesicle; 9 = trigeminal ganglia; 10 = otic capsule. Structures
are based on previous descriptions of Xenopus neuroanatomy [16,17,25]. Probe preparation and whole-mount in situ hybridization were performed as