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gap43xenopus midbrain [+] 

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Experiment details for gap43

Cloning and embryonic expression of Xenopus laevis GAP-43 (XGAP-43).

Cloning and embryonic expression of Xenopus laevis GAP-43 (XGAP-43).

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Gene Clone Species Stages Anatomy
gap43.L laevis NF stage 33 and 34 midbrain

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  Fig. 4. Whole-mount in situ hybridization of XGAP-43 during Xenopus laevis embryogenesis. Numbers in the left column represent the approximate stage of embryos in each row. Embryos in columns (A), (B), (D) and (E) were incubated with a full-length antisense XGAP-43 probe and those in columns (C) and (F) with full-length sense XGAP-43. Lateral views of XGAP-43 expression are shown in columns (A) and (D-F), and dorsal views in column (B). XGAP-43 transcripts were first observed around stage 22 where they appeared as two parallel stripes on either side of the midline, and also in bilateral regions of the prospective forebrain/midbrain border. In later stages, XGAP-43 expression was observed in a complex spatiotemporal pattern throughout the nervous system. E: magnification of CNS structures expressing XGAP-43 in a stage 34 embryo: 1 = brainstem; 2 = hindbrain; 3- cerebellum; 4 = midbrain; 5 = pineal gland; 6 = forebrain (olfactory region); 7 = diencephalon; 8 = optic vesicle; 9 = trigeminal ganglia; 10 = otic capsule. Structures are based on previous descriptions of Xenopus neuroanatomy [16,17,25]. Probe preparation and whole-mount in situ hybridization were performed as described [26].