| |
Fig. 4. XStau and Vg1 RNA are colocalized in Xenopus oocytes. (A-D) Stage III-IV oocytes were microinjected with Alexa-546 labeled VLE RNA, followed by immunofluorescence using XStau antibodies and Alexa-647 secondary antibodies. (A) Localization of endogenous XStau, shown in red. (B) Injected VLE RNA, shown in green. (C) Colocalization of endogenous XStau and injected VLE RNA, digitally represented in white. (D) High-magnification image of the oocyte shown in C. (E) High-magnification image of the oocyte shown in A. (F) Distribution of XStau in vegetal hemisphere of an uninjected stage III oocyte, determined by immunofluorescence using XStau antibodies and Alexa-568-conjugated secondary antibodies. For all panels, representative confocal sections are shown. Scale bars: 20 μm. |
