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Fig. 4. In situ hybridization of coronal sections through the sensory
epithelium of the medial and lateral diverticulum of Xenopus
using Ga subtype-specific antisense RNA probes. Coronal sections
were hybridized with DIG-labeled antisense RNA specific for Gasx2
(A,B) and Gaox1 (C,D). Hybridization with the Gasx2-specific probe
intensely labeled the olfactory sensory cell layer (A), whereas hybridization
signals in the lateral diverticulum are low (B). Hybridization
with the Gaox1-specific probe led to very few hybridization signals in
the medial diverticulum (C), whereas many sensory cells in the lateral
diverticulum were labeled (D). oe, olfactory neuron layer; sc,
sustentacular cells; bs, basal cells; lp, lamina propria; bg, Bowman‘s
glands. Scale bar 5 20 mm. |
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Fig. 5. Immunohistochemical localization of G protein a subtypes
in the olfactory epithelium of the medial and lateral diverticulum.
Cryostat sections of the medial (A) and lateral diverticulum (B) of the
Xenopus nasal cavity were probed with a 1:1000 dilution of a Gas/olf -
or Gao1-specific antibody. Note that the immunoreactivity detectable
for the Gas/olf -specific antibody is restricted to the cilia layer of the
sensory epithelium (se); at the transition area toward the respiratory
epithelium (re), indicated by an arrowhead, immunoreactivity disappeared.
mp, melanophores. Scale bar 5 10 mm. |
