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Fig. 4. Expression of G-proteins in the vomeronasal organ (VNO),
and expression of the Xenopus V2R in the principal cavity (PC) and
the middle cavity (MC) of the nasal capsule. VNO cross-sections were
hybridized to Go (A) and Gi2 (B) antisense probes, and to Go (C) and
Gi2 (D) sense probes (negative controls). Some cells in the MC were
hybridized to the Gi2-antisense probe (B’) (positive control for the
Gi2-antisense probe). The antisense Xenopus V2R probes derived
from clones A-1, C-1, and E-1 were mixed, and sections of Xenopus PC
(E) and MC (F) epithelia were hybridized to these probes. An arrow
indicates the V2R-expressing cell (F). Scale bars 50 m. |
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Fig. 5. Expression of Xenopus
V2Rs in the posterolateral epithelial
area of the principal cavity.
Photographs of hematoxylinstained
transverse sections of Xenopus
laevis upper jaw at intervals
of 400 m (A). Arrow indicates the
V2R-expressing region (A-3). PC,
Principal cavity; MC, middle cavity;
VNO, vomeronasal organ.
Cross-sections of Xenopus nose
were hybridized to digoxigeninlabeled
antisense probes prepared
from clones E-1 (B), xV2R1 (C),
and Go (D). Arrows indicate the
xV2R1-expressing cells (C). Scale
bars 1 mm in A; 100 m in B–D. |
