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h3-5xenopus neuron 

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Experiment details for h3-5

Zivraj KH et al. (2010) Assay

Subcellular profiling reveals distinct and developmentally regulated repertoire of growth cone mRNAs.

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Gene Clone Species Stages Anatomy
h3-5.L laevis NF stage 32 neuron , retinal ganglion cell

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  Figure 4. FISH validation of mRNAs in growth cones. A , Quantitative FISH (see Materials and Methods) was performed on stage 32 Xenopus growth cones to obtain the mean pixel (fluorescence) intensity/unit area of antisense signal. Corresponding sense probes were used as controls for signal specificity, and all antisense mean pixel intensity/unit area were normalized to the sense control. The mRNAs chosen sample broadly across the different functional categories identified by the microarray analysis. Transcripts validated include ephrin A3 ( C ) (secreted and ECM category); eIF5A ( D ), Ribosomal protein S13 ( E ), Cyclophilin A ( F ), and Splicing factor 3A ( G ) (protein synthesis and translation category); Ubiquitin C ( H ) (protein degradation and apoptosis category); Histone H3 ( I ) (nuclear category); Solute carrier member 25 ( J ) and Ferritin ( K ) (metabolic/glycolytic category); Thymosin β4 peptide ( L ) and α-tubulin ( M ) (cytoskeletal/motor category); and 67 kDa Ribosomal protein/Laminin receptor 1 ( N ) and Vacuolar sorting protein 4A ( O ) (transmembrane/cell surface receptor and membrane trafficking categories, respectively). GAPDH mRNA was absent in growth cones and served as a negative control ( B ). Scale bar, 5 μm. Insets in boxed areas at higher magnification show differences in granule size and density (scale bar in E inset, 0.5 μm). [Xenbase curators note: accession number given for panel O matches sntb2 gene in NCBI/Genbank- curated as this gene here)