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Figure 6. . Thrombopoietin (TPO) augments early hematopoiesis in Xenopus embryos. The embryos injected with tpo mRNA into the ventral marginal zone at the 4-cell stage were developed to stage 35/36 and were analyzed as follows. (A,B) Whole-mount o-dianisidine staining of the embryos processed as described in Materials and Methods. Brown o-dianisidine-positive cells indicate erythrocytes. (C–H) The sections of embryos labeled with an asterisk in (A) and (B) were analyzed by immunostaining using the L5.41 antibody. (C,D) Transverse sections of the region anterior to the heart indicated by an arrow in (A). Bar, 100 μm. (E,F) Transverse sections of ventral blood island (VBI). Bar, 25 μm. (G,H) Transverse sections of the dorsal–lateral plate (DLP) region. Bar, 50 μm. (I,J) The sections of embryos labeled with an asterisk in (A) and (B) analyzed by immunostaining using the XL-2 antibody. (I,J) Transverse sections of the DLP region. Bar, 50 μm. (A,C,E,G,I) Rat tpo mRNA (100 pg) injected embryo. (B,D,F,H,J) An uninjected embryo. Arrow and arrowheads indicate immunostaining on hematopoietic cells. Abbreviations: ao, aorta; b, brain; gl, glomus; me, mesonephros; ph, pharynx; n, notochord; s, somite. |
