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Figure 7. . Ectopic expression of thrombopoietin (TPO) promotes the heterotopic erythropoiesis in the head region of Xenopus embryos. The embryos injected with tpo mRNA into the ventral marginal zone at the 4-cell stage were developed to stage 35/36 and analyzed by immunostaining using the L5.41 antibody. Brown cells indicate immunostaining on erythrocytes. The transverse sections of uninjected embryos are shown in (A–C) and rat tpo mRNA (100 pg) injected embryos in (D–F). Bar, 250 μm. (G–I) shows enlargements of (D–F). Bar, 50 μm. Abbreviations: b, brain; n, notochord; ph, pharynx; s, somite. |