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FIG. 1. (A) Whole embryo in 8itu analysis using an antisense digoxigenin-labeled larval a-globin RNA probe. a-Globin is not detected at stage
22 ( t.op embryo). The second and third embryos are ventral and lateral views, respectively, at stage 26 showing a-globin RNA to be expressed in
the ventral region in a characteristic V shape. The fourth embryo. stage 32, demonstrates the boundat·ies of the blood island-expressing C~-globin
RNA. At stage 41 globin is seen throughout the circulation. (B) Whole embryo immunohistochemistry using monoclonal antibody L4-27 against
larval globins; stage 32 embryo. (C) Cross section of stage 32embryo immunohistochemically stained with the L4-27 antibody. (D) Whole embryo
(stage 32} i11 situ analysis using an antisense digoxigenin-labeled GATA-1 RNA probe. |
