| |
Fig. 5. Effect of animal pole
tissue on a-globin expression
in control and exogastrula
embryos. Treated embryos
(B) and untreated embryos
(A) were allowed to
develop until stage 7 and then
cultured in 1.4å~MMR as described
in Materials and Methods.
Such embryos were further
cultured for 2 days (stage
35/36) and were used for
whole-mount in situ hybridization
analysis (A,B) and
Northern blotting analysis (C)
to detect the expression of a-
globin. In exogastrula embryos (B), no expression of a-globin was observed. Scale bar in B indicates 2 mm. In addition, the expression of a-actin was
not affected in the exogastrula embryos. The expression of EF-1a served as a loading control. |
