| |
FIG. 6. Ruby dextran detects more progeny than Lac Z mRNA
when coinjected into blastomere C3. St. 32 embryos were stained
for Lac Z activity (red), probed by in situ hybridization for globin
expression (turquoise), and stained for ruby dextran (brown). Lac Z
staining varies between embryos (A), while globin expression and
ruby dextran staining appear fairly consistent. Most noticeable is
the detection of many progeny of C3 in the lateral plate mesoderm.
In (B), both red nuclei and brown cytoplasmic staining detect C3
progeny overlapping with globin expression in the VBI. In (C),
C3-derived, Lac Z-positive cells are among the globin-positive cells
in the VBI. Numerous dextran-positive, Lac Z-negative (brown cells
without red nuclei) are visible in the sectioned embryo (C, D), and
are present in the VBI. The contralateral, uninjected side of the
embryo serves as a staining control. |
