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FIG. 4. In situ hybridization of cloned globin cDNAs to erythroblasts
from anemic tadpoles at stage 59. Blood smears from anemic tadpoles
at stage 59 were hybridized as described under Materials and Methods
using aH nick-translated inserts from individual globin cDNA clones.
Exposure time was 18 days. (A) Mixture of cuk, /3!, af, and j$’ probes.
(B) Mixture of (Y: and & probes only. |
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FIG. 8. Presence of globin mRNA in a mitotic erythroblast. Cells
of anemic larvae at stage 59 were hybridized in situ as described under
Materials and Methods with a mixture of 32P nick-translated insert
of (Y: and (3: clones. Exposure time was 18 days. |
