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hes2xenopus   

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Experiment details for hes2

Dissecting the pre-placodal transcriptome to reveal presumptive direct targets of Six1 and Eya1 in cranial placodes.

Dissecting the pre-placodal transcriptome to reveal presumptive direct targets of Six1 and Eya1 in cranial placodes.

Gene Clone Species Stages Anatomy
hes2.L laevis NF stage 18 preplacodal ectoderm , anterior placodal area , posterior placodal area

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  Figure 4—figure supplement 5. Expression of selected targets in pre-placodal ectoderm (PPE) in sections through neural plate stage Xenopus embryos. Neural crest and (NC) and neural plate (NP) domains are indicated. (A–D): Hes2 (A), Hes8 (B), Hes9 (C) and Pou4f1.2 (D) are all expressed in parts of the PPE (for level of sections see Figure 4 —figure supplements 3 and 4). While the exact boundaries of NC and NP cannot be determined in these sections, comparisons with sections through embryos stained by double in-situ-hybridisation for the PPE marker Six1 and the NP marker Sox3 (E) or Six1 and the NC marker FoxD3 (F) indicate that expression of each of these target genes is largely confined to the PPE although some overlap with the lateral NC region cannot be ruled out (E and F modified from Schlosser and Ahrens, 2004; Figure 6). Bar in A: 100 μm (also for B–F).