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Figure 3. Spatial analysis of endogenous xHIF-1 alpha expression in the Xenopus embryo. A: A representative whole-mount in situ hybridization of a stage 35 embryo using a digoxigenin-labeled xHIF-1 alpha antisense probe showing a ubiquitous expression pattern. B: Elevated expression is seen in the head, somitic tissue, and developing nervous system when compared with stage 35 embryos hybridized with a complementary sense probe. C-F: The observed expression pattern was confirmed through histological analysis of embryos hybridized with either the xHIF-1 alpha antisense (C,E) or sense (D,F) probe. NO, notochord; NT, neural tube; OV, otic vesicle; SO, somite; arrowheads indicate somitic tissue; an asterisk also indicates the otic vesicle. Scale bars = 1 mm in A,B, 0.5 mm in C-F. |
