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FIGURE 1. Expression of hif-1 during Xenopus development. A, predicted
domains of Xenopus HIF-1 . bHLH, basic helix loop helix binding domain; PAS,
Per-Arnt-Sim domain; TAD-N, N-terminal activation domain; TAD-C, C-terminal
activation domain. B, temporal expression profile of the hif-1 mRNA.
RT-PCR analysis was carried out from stage 1 to 25 using a primer set for
hif-1 . Quality of RNA at each stage was assessed by amplification of the
ubiquitous transcript, ornithine decarboxylase (odc). C, hif-1 expression was
examined by whole mount in situ hybridization of embryos at the stage indicated
in each panel. a, lateral view of stage 23 embryo; b, lateral view of stage
28 embryo; c– e, Stage 33/34 embryos, lateral view (c), ventral view (d), and
higher magnification image (e) of the anterior portion seen in panel d. hif-1
expression in the eyes (yellow arrowhead), brain (yellow arrow), kidneys (red
arrow), somites (yellow asterisk), branchial arches (black arrowhead), myocardium
(red arrowhead), and the ventral blood island (VBI, black asterisk). f, transversal
section of a stage 33/34 embryo at the white line in panel d reveals
hif-1 expressed in myocardium. |
