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Fig. 4. Spatial expression of RHAMM mRNA in proliferating cell populations. (A) Cryostat horizontal section at the branchial level of a stage 37 embryo showing RHAMM mRNA expression in cranial NCC migrated into the branchial pouches (I, II, III, IV). (B) Adjacent section showing the proliferating cells marker cyclin D1 mRNA expression for comparison. (C) Cryostat horizontal section at eyes level, at stage 37, showing that RHAMM expression in the eye is restricted to the CMZ (arrow) and in the ventricular region of the neural tube (arrowhead). (D) Adjacent section showing the cyclin D1 mRNA expression for comparison. (C′–D′) Magnification of the embryos in (C) and (D) showing the label in the ventricular zone (arrowheads). (C′′–D′′) Magnification of the embryos in (C) and (D) showing the label in the CMZ (arrows). (E) Magnification of cryostat horizontal section at eyes level of a stage 40 embryo showing that RHAMM transcription is restricted to the CMZ (arrow) close to the lens (L). (F) Adjacent section showing the cyclin D1 mRNA expression for comparison. |
