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Fig. 1. Homez structure and gene expression during embryogenesis. (A) Shematic representation of the Homez protein. HD, homeodomain; L, leucine repeats, A, acidic
domain. (B) Temporal expression of Homez by RT-PCR. RNA was extracted from embryos at the indicated stages. (C) RT-PCR analysis of Homez expression in dissected explants
of stage 10.5 embryos. The endodermal VegT, mesodermal Xbra and ectodermal Xema markers were used as dissection controls. In B and C, Histone H4 was used as a loading
control. -RT, control RT-PCR without reverse transcriptase. (D-I) Spatial expression of Homez compared to that of Ngnr1, Myt1 and N-tubulin analyzed by whole-mount in situ
hybridization. Nieuwkoop-Faber stages are indicated. (D–H) Dorsal views. (I) Lateral view. Stripes of Homez expression in the domains of primary neurogenesis in the
posterior neural plate are indicated (l, lateral; i intermediate; m, medial). Note that Homez is activated in the domains of primary neurogenesis at E12.5 when N-tubulin is not
yet detectable. (J–M) Homez expression compared to that of Ngnr1, Myt1 and N-tubulin in the neural tube of stage 32 embryos. Sections at the level of the otic vesicle are
shown. Note that XHomez+ cells are detected more medially than N-tubulin in the marginal zone. Abbreviations: AC, animal caps; ba, branchial arches; cg, cranial ganglia; e,
egg; CE, control embryo; MZ, marginal zone; VP, vegetal pole. |
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Fig. 2. Homez is regulated positively by attenuation of Bmps and Ngnr1 and
negatively by Notch. (A) RT-PCR analysis of Homez expression in animal cap
explants isolated from embryos injected with increasing doses of noggin mRNA or
from uninjected control animal caps (CC). CE, control embryos. Ep. Keratin and the
pan-neural Sox3 gene were used as controls for neural induction. Histone H4 was
used as a loading control. -RT, control RT-PCR without reverse transcriptase. (B–D)
In situ analysis of Homez expression in neurula embryos (dorsal views) injected
unilaterally with the indicated mRNA and LacZ mRNA. Injected sides (IS) are
indicated. |
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homez (homeobox and leucine zipper encoding) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior right, dorsal up. |
