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Fig. 6. Analysis of the effects of X-CypA over-expression on the expression of neural markers by in situ hybridisation. Embryos were co-injected into one cell of the 2-cell stage with 0.152, 0.076 or 0.038â ng X-CypA and 0.8â ng GFP mRNAs and cultured to stage 19. The embryos were then subjected to in situ hybridisation using En2, HoxB9, Krox20, Slug probes. No obvious effects on the expression of En2 or HoxB9 were observed. However, the expression of Krox20 and Slug were affected by the misregulation of X-CypA. Extension and disorganisation of the neural crest domain of Krox 20 is noticeable (compare Jâ L to I), the expression of Slug mRNA is increased on the injected side (compare M to Nâ P). An asterisk indicates the injected side. Arrowheads indicate altered expression pattern. |
