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Fig. 2. XMeis3 and RA cooperatively regulate HoxB3 expression. Two-cell albino embryos were injected unilaterally into the animal hemisphere of one blastomere. All embryos are injected on the right side, viewed dorsally, and are oriented anterior (top), posterior (bottom). (A) Control (uninjected; HoxB3 and HoxB9 staining). (B) 0.8 ng XMeis3 RNA (HoxB3 and HoxB9 staining) HoxB3 expression is increased in 44% of the embryos (n = 18/41). (C) 7.5 ng of XMeis3 MO (only HoxB3), HoxB3 expression is reduced in 73% of the embryos (n = 25/34). (D) 0.1 AM RA (uninjected; only HoxB3) HoxB3 expression is anteriorly expanded in 94% of the embryos (n = 33/35). (E) 0.8 ng XMeis3 RNA; 0.1 AM RA (HoxB3 and HoxB9 staining), HoxB3 expression is highly increased in 67% of the embryos (n = 22/33). (F) 7.5 ng XMeis3 MO; 0.1 AM RA (only HoxB3), HoxB3 expression is highly reduced in 92% of the embryos (n = 34/37). (G) 0.8 ng XCYP26 RNA; 50 pg h-gal RNA (only HoxB3), HoxB3 expression is posteriorized in 68% of the embryos (n = 13/19). (H) 0.8 ng XCYP26 RNA; 0.8 ng XMeis3 RNA; 50 pg h-gal RNA (only HoxB3), HoxB3 expression is reduced in 82% of the embryos (n = 23/28). |
