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Fig. 5. Phenotype of xBtg-x antisense morpholino oligomer injections. (A) In vitro transcription/translation of pCS2-xBtg-x in the presence of control morpholino and xBtg-x-MO1. (B) Histological analysis of uninjected control embryos and (C) embryos injected at the 2-cell stage with xBtg-x-MO1 + 2 at stage 40; en, endoderm; nt, neural tube; no, notochord; so, somites. (D�K) Whole-mount in situ hybridization of uninjected and xBtg-x-MO1 + 2 injected embryos at neurula stage 14. (D, E) Combination of Otx2, Krox20, and HoxB9 probes; (F, G) Sox-2; (H, I) Pax3; (J, K) Slug. Note that xBtg-x-MO1 + 2 inhibits neural plate convergence and extension movements.
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