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Fig. 1. Knockdown of Coco causes anterior truncations at tadpole stages. (A-H) Xenopus embryos at stage 28. Overexpression of Coco results in embryos with an ectopic head; compare uninjected embryo (A) with Coco-injected (B, asterisk). Knockdown of Coco causes anterior truncations (C). Hoxb9 is expressed in the spinal cord (D, arrow); Coco morphant embryos have no expression of Hoxb9 (E). Host transfers were also performed with MO (F-H). Embryos have a loss of anterior structures (G, arrowheads, compared with control embryo in F). Anterior structures, such as the cement gland (H, arrowheads) could be rescued by injection of Coco RNA. (A,B) Lateral view, anterior to the left. (C-H) Anterior to the top. cg, cement gland; fb, forebrain; hb, hindbrain; mb, midbrain; sc, spinal cord. |
