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Fig. 9. Repression of xBMP-3b and cerberus leads to impaired gastrulation
movements. Lineage of cells targeted by MOs and expression of
marker genes were monitored at gastrula, neurula and tadpole stages.
(Left) Embryos injected with MOs for both xBMP-3b and cerberus.
(Right) Control embryos injected with globin MO. Light blue staining
represents cells targeted by MOs. Brown staining represents expression
of markers as indicated on right corner. Dorsal side faces top in panels
of gastrula and tadpole, and anterior side faces top in panels of neurula.
Injection of MOs for xBMP-3b and cerberus did not affect dorsal lip
formation and expression of organizer genes at beginning of gastrulation,
but led to delayed blastopore closure and downregulation of Otx2
expression by neurula stage. Consequently, suppressing xBMP-3b and
cerberus functions elevated expression of a ventral–posterior marker,
Xcad1. |
