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FIG. 3. Whole and half organizers were wrapped in late stage 9/early stage 10 animal cap ectoderm (wraps) and examined for expression
of otxA (blue) and Hox B9 (pink) by whole-mount in situ hybridization. (A) A normal tadpole at stage 25 expresses both genes; (B) isolated
animal caps never express otxA and occasionally express Hox B9 (not shown; see Table 1); (C) whole organizers induce both genes; (D)
anterior organizers induce otxA but no significant Hox B9; and (E) posterior organizers induce both genes. |
