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Figure 4 Photomicrographs of sections through two Stage 41 Xenopus retinas, each of which
received a transplant of optic vesicle tissue from a younger, dextran-FITC labeled embryo. In both
instances the host embryo was at Stage 28, and the donor at Stage 24 at the time of transplant. The
host embryos were allowed to grow to a stage when the retina was mature, that is, when both graft
and host tissue were at Stage 41. The sections are counterstained with bis-benzimide, which
demonstrates that the retinal cytoarchitecture develops normally following the transplant surgery.
The graft survives and is apparent as a wedge of green labeled cells well integrated into the host
retina and with normal cytoarchitecture. Finally, the sections are immunostained with XAP-1 (A)
and XAP-2 (B) antibodies (red), which are normally expressed, in both cases, in the host and the
graft. Thus, the younger graft of a heterochronic transplant is seen to eventually express XAP-1 and
-2, but to do so on its own schedule. Scale bar: 50 mm. |
