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Fie. 8. Spatial pattet·ns of int.lgrin {33 mRNA expression analyzed by whole mount in situ hybridization. Whole n10unt in situ hybridizations
reveal a more restricted pattern of integrin 13, mRNA expression in comparison to that of /31 • (A) Side view of a stage 17 neurula; note staining in
a line of cells in the neural groove at the anterior end (arrow). (B) Dorsal view of the same embryo in (A), staining in the neural groove (arrow)
and neural folds (arrowheads). (C) Dorsal view of a stage 18 neurula; {J3 mRNA expression in the neural groove (arrow) decreases beginning at
the posterior end of the embryo and proceeding anteriorly. Arrowheads indicate staining along the neural folds. (D) Side view of a stage 30
embryo that has begun to express /33 mRNA in a subset ofventral blood island cells (arrow). (E) Side view of a stage :32/33 tailbud embryo before
the onset of blood circulation; note the broadly distributed mass of stained ventral cells (arrows). (F} Stage 33/34 embryo after circulation has
begun. The /13-positive cells are now found scattered throughout the embryo. (G, H) Stage 35/36 and 37/38 embryos have an increased number of
cells expressing Ps associated with major blood vessels. (H) Note the line of stained cells along dorsal blood vessels (arrowheads) and in the
regi()n o£ the heart (arrow}. |
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itgb3 ( integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 30, lateral view, anterior left, dorsal up. |
