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Fig. 7. Voltage-dependent potassium (Kv) subunits are differentially
expressed in developing Xenopus retinal ganglion cell (RGC)
growth cones. Stage 24 eye primordia were grown in culture for 24–48
hours and labelled with various Kv subunit antibodies. A: Phase
micrograph of the same RGC axons shown in B. Arrowhead points to
a filopodia. B: The Kv1.3 channel antibody labels the axon abundantly,
but not the body of the growth cone, nor the filopodia or
lamellipodia. C: The Kv1.5 antibody labels the RGC axon and the
entire growth cone, including the filopodia (arrowhead). D: The Kv4.2
antibody labels the RGC axon and growth cone in a punctate manner.
Scale bar 16 m for A–D. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
isl1
|
|
laevis
|
NF stage 33 and 34
|
retina
,
retinal ganglion cell layer
,
retinal ganglion cell
|
actb
|
|
laevis
|
NF stage 33 and 34
to
NF stage 40
|
retinal ganglion cell
,
growth cone
,
axon
|
kcnd3
|
|
laevis
|
NF stage 33 and 34
|
retina
,
retinal ganglion cell layer
,
retinal ganglion cell
|
kcnd3
|
|
laevis
|
NF stage 41
|
optic nerve
,
retinal outer plexiform layer
,
retinal inner plexiform layer
,
axon
,
optic disc
|
kcnc4
|
|
tropicalis
|
NF stage 33 and 34
to
NF stage 40
|
retinal ganglion cell
,
growth cone
,
axon
|
|
|
Fig. 3. Voltage-dependent potassium (Kv) subunit-like immunoreactivity
in developing retinal ganglion cells (RGCs) and their axons.
A: Double labelling of a stage 33/34 retina with anti-Kv1.3 (green) and
mouse anti-bromodeoxyuridine (BrdU; red) antibodies shows that in
the central retina Kv1.3-like immunopositive cells (arrows) are not
BrdU positive. B: Double labelling (yellow) with antibodies against
the rat Kv4.2 subunit and islet-1 at stage 33/34. The islet-1 antibody
stains all RGCs (red), but the Kv4.2 antibody (green) labels only a
subset of these islet-1–positive RGCs. C: Double labelling (yellow) of
a stage 41 retina with a Muller glial marker (3CB2) and the anti-
Kv4.2 antibody shows that radial cell processes in the central third of
the retina are immunopositive (arrows) for both markers, whereas
processes in the peripheral retina are immunopositive for the Kv4.2-
like subunit only (arrowheads). By this stage the optic nerve head
(onh), but not RGC somata, are Kv4.2-like immunopositive.
D: Merged image of E and F shows colocalization of the Kv3.4 subunit
and actin filaments in an RGC growth cone. E: Actin labelled with
rhodamine phalloidin. F: The Kv3.4 antibody labels the axon and
growth cone. IPL, inner plexiform layer; L, lens; OPL, outer plexiform
layer; RGCL, RGC layer. Scale bar 100 m for A–C and 10 m for
D–F. |