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kcna3xenopus   

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Experiment details for kcna3

Pollock NS et al. (2002) Assay



Gene Clone Species Stages Anatomy
kcna3.S laevis NF stage 24 retinal ganglion cell , axon

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  Fig. 7. Voltage-dependent potassium (Kv) subunits are differentially expressed in developing Xenopus retinal ganglion cell (RGC) growth cones. Stage 24 eye primordia were grown in culture for 24–48 hours and labelled with various Kv subunit antibodies. A: Phase micrograph of the same RGC axons shown in B. Arrowhead points to a filopodia. B: The Kv1.3 channel antibody labels the axon abundantly, but not the body of the growth cone, nor the filopodia or lamellipodia. C: The Kv1.5 antibody labels the RGC axon and the entire growth cone, including the filopodia (arrowhead). D: The Kv4.2 antibody labels the RGC axon and growth cone in a punctate manner. Scale bar 16 m for A–D.

Gene Clone Species Stages Anatomy
kcna3.S laevis NF stage 33 and 34 retina , ciliary marginal zone

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  Fig. 3. Voltage-dependent potassium (Kv) subunit-like immunoreactivity in developing retinal ganglion cells (RGCs) and their axons. A: Double labelling of a stage 33/34 retina with anti-Kv1.3 (green) and mouse anti-bromodeoxyuridine (BrdU; red) antibodies shows that in the central retina Kv1.3-like immunopositive cells (arrows) are not BrdU positive. B: Double labelling (yellow) with antibodies against the rat Kv4.2 subunit and islet-1 at stage 33/34. The islet-1 antibody stains all RGCs (red), but the Kv4.2 antibody (green) labels only a subset of these islet-1–positive RGCs. C: Double labelling (yellow) of a stage 41 retina with a Muller glial marker (3CB2) and the anti- Kv4.2 antibody shows that radial cell processes in the central third of the retina are immunopositive (arrows) for both markers, whereas processes in the peripheral retina are immunopositive for the Kv4.2- like subunit only (arrowheads). By this stage the optic nerve head (onh), but not RGC somata, are Kv4.2-like immunopositive. D: Merged image of E and F shows colocalization of the Kv3.4 subunit and actin filaments in an RGC growth cone. E: Actin labelled with rhodamine phalloidin. F: The Kv3.4 antibody labels the axon and growth cone. IPL, inner plexiform layer; L, lens; OPL, outer plexiform layer; RGCL, RGC layer. Scale bar 100 m for A–C and 10 m for D–F.