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kcnj10xenopus   

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Experiment details for kcnj10

Yaoita Y and Nakajima K (2018) Assay

Developmental gene expression patterns in the brain and liver of Xenopus tropicalis during metamorphosis climax.

Gene Clone Species Stages Anatomy
kcnj10 tropicalis NF stage 54 to adult frog stage brain

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  Figure 1 Developmental expression of neural tissue-related genes in the X. tropicalis brain. qRT-PCR was conducted using total cellular brain RNA and gene-specific primers (Supporting Information Table S2). The relative mRNA expression level of the indicated gene to EF1 mRNA is presented. Data are expressed as mean +/-SD (biological replicate number = 57)

Gene Clone Species Stages Anatomy
kcnj10 tropicalis NF stage 61 brain

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  Figure 3 Expression of neural tissue-related and synapse-related genes in the brain of NF stage 61 wild-type and TR-knockout tadpoles. Total cellular RNA was extracted from brains of NF stage 61 wild-type, TR-knockout, and TR-knockout tadpoles, and qRTPCR was conducted using gene-specific primers (Supporting Information Table S2). The relative mRNA expression level of the indicated gene to EF1 mRNA is presented. Data are expressed as mean SD. Biological replicate numbers of wild-type, TR-knockout, and TR-knockout brain samples are five, five, and four, respectively. Statistical significance by comparison of TR-knockout tadpoles with wild-type and TR-knockout tadpoles were assessed using the Tukey's test. *p < 0.05, **p < 0.01