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mapk6xenopus   

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Experiment details for mapk6

Takahashi C et al. (2018) Assay

The atypical mitogen-activated protein kinase ERK3 is essential for establishment of epithelial architecture.

Gene Clone Species Stages Anatomy
mapk6.L laevis NF stage 9 animal hemisphere
mapk6.L laevis NF stage 12 ectoderm , animal cap
mapk6.L laevis NF stage 19 epidermis , neural crest , somite , neural tube
mapk6.L laevis NF stage 25 to NF stage 33 and 34 brain , epidermis , neural crest , somite , eye , [+]

  Figure 1. ERK3 expression in Xenopus laevis embryos. A, Real-time quantitative RT-PCR analysis. The expression levels of ERK3 were normalized to those of odc in two independent experiments (#1 and #2). The normalized ERK3 expression level at stage 1 was defined as 1.0 in each experiment. B, Whole-mount in situ hybridization analysis of ERK3 expression. ec, ectoderm; nc, neural crest; s, somite; nt, neural tube; ep, epidermis; pn, pronephros; b, brain; e, eye. Scale bars, 400 m. Anterior is to the left (stage 19-33/34). Dorsal is up for the lateral views (stage 19-33/34). Shown are representative images of 6-10 embryos from one experiment using the ERK3A probe. Essentially the same results were obtained for 6-9 embryos from another experiment using the ERK3B probe.

Gene Clone Species Stages Anatomy
mapk6.L laevis NF stage 12 ectoderm , animal cap

  mapk6 (mitogen-activated protein kinase 6) gene expression in X. laevis embryo, as assayed by in situ hybridization, NF stage 12, ventral view.

Gene Clone Species Stages Anatomy
mapk6.L laevis NF stage 19 epidermis , neural crest , somite , neural tube

  mapk6 (mitogen-activated protein kinase 6) gene expression in X. laevis embryo, as assayed by in situ hybridization, NF stage 19, lateral view, anterior left, dorsal up (left image), and dorsal view, anterior left (right image).

Gene Clone Species Stages Anatomy
mapk6.L laevis NF stage 25 brain , epidermis , somite , eye , neural tube

  mapk6 (mitogen-activated protein kinase 6) gene expression in X. laevis embryo, as assayed by in situ hybridization, NF stage 25, dorsal view, anterior left.

Gene Clone Species Stages Anatomy
mapk6.L laevis NF stage 25 brain , spinal cord , epidermis , neural crest , eye , [+]

  mapk6 (mitogen-activated protein kinase 6) gene expression in X. laevis embryo, as assayed by in situ hybridization, NF stage 25, lateral view, anterior left, dorsal up.

Gene Clone Species Stages Anatomy
mapk6.L laevis NF stage 33 and 34 brain , epidermis , neural crest , somite , eye , [+]

  mapk6 (mitogen-activated protein kinase 6) gene eexpression in X. laevis embryo, as assayed by in situ hybridization, NF stage 33 & 34, lateral view, anterior left, dorsal up.