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Figure 2. Association of ST3 expression with larval epithelial cell death during intestinal remodeling. Stages 56 (A, B, C) and stage 60 (D, E, F) intestines were analyzed by in situ hybridization for ST3 expression (A, B, D, E) and by TUNEL assays for apoptotic cell detection (C, F). There was no detectable ST3 expression at stage 56 (B). ST3 expression was strong in all regions of the connective tissue at stage 60 but not in the epithelium or muscles (E). Likewise, at stage 56, there was no apoptotic signals (C), while there was many apoptotic signals, mainly within the epithelium, at stage 60 (arrows). Note that the control hybridization with the sense RNA probe did not detect any signal, as expected. ct, connective tissue; e, epithelium; l, lumen. White bars: 250 um. |
