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Fig. 7. Cross-sections of Xhl whole-mount labelled embryos. (a) Section through the posterior part of a stage 12 embryo. Arrows indicate Xhl positive
cells. Xhl mRNA is localized in epithelial and deeper cell layers of the posterior part of the notoplate. (b) Rosin-stained section through a more anterior
part of the same embryo as in (a). Arrows mark epithelial cells expressing Xhl mRNA. Bar indicates the midline of the prospective neural plate. (c)
Section through the posterior part of a stage 13 embryo showing that Xhl mRNA is localized mainly in epithelial cells of the notoplate. Arrow points at
cells of deeper cell-layers expressing Xhl mRNA. Bar marks the region of the notoplate. (d) In more anterior parts of stage 13 embryos Xhl-expressing
cells are exclusively localized in epithelial cells of the notoplate which ate indicated by arrows. (e) Cross-section of a stage 15 embryo showing bottlecells
(arrow) expressing Xhl mRNA. (f) Higher magnification of section shown in (e). Arrow points at neural groove and bottle-cells, respectively.
Since Xhl represents a low-abundant mRNA, only weak signals were observed in cross-sections of whole-mount labelled embryos, Longer staining of
embryos, however, resulted in mom background staining, making it difficult to distinguish specific from unspecific signals. yp, yolk plug; ae, archenteron;
n, notochord; s, somitic mesoderm. |
