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Fig. 2. Expression of Livertine in the neural ectoderm of gastrula and neurula stage frog embryos. (A-C) Whole-mount in situ hybridization pictures of
the expression of Livertine in early gastrula (stage -10.5-11; A), late-gastrola (stage -12.5; B) and late gaswala/early neurula (stage -13-14; C) frog
embryos. In all cases dorsal side is up and arrows point to the sites of expression. Note the decreasing size of the blastopore (bp) during gastrulation, a,
anterior; p, posterior; md, midline neural plate (np) cells. (D,E) Expression of L/vert/ne in the superficial layer of the neural plate seen in a side view of
a whole-mount (D) at stage 11-11.5 and in cross section through the anterior region of a stage -14 embryo (E). Arrows point to sites of expression and
the brackets show the position of the different tissues, bp, blastopore; d, deep layer of the neural plate; en, endoderm of the archenteron (ar) roof; me,
axial mesoderm; n, notochord; ne, neural ectoderm; s, superficial layer of the neural plate; so, somite. (F) Dorsolateral view of the expression of Livertine
in the ectoderm (ec) of an early exogastrula (stage -15) in cells occupying the position characteristic for the notoplate, a, anterior in the ectoderm;
m, mesoderm; p, posterior in the ectoderm. Scale bar: (A,B,C,F) 240tim; (D) 60/~m; (E) 100#m. |
