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Fig. 4. Ultrastructural analysis of oocytes on COX activity during oogenesis. (A′–J′) KCN inhibits COX activity in all stages of oocytes. Dense granules (arrowheads) are mitochondrial
granules, not reaction products. (A, B) No mitochondria in stage I oocytes showed COX activity. (C, D) All mitochondria in early stage II oocytes showed high COX activity. (F, H, J) COX
activity was heterogeneous in the population and also in areas of one mitochondrion at the vegetal hemisphere of stage III oocytes (F). Mitochondria at the vegetal cortex showed noCOX activity in stage IV (H) and VI oocytes (J). (E, G, I) Mitochondria in the animal hemisphere showed high COX activity. Scale bars represent 200 nm. |