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Figure 1. Mel1a and Mel1b receptors are differentially distributed in the outer retina. A: Mel1a immunoreactivity (red) in the outer plexiform layer (OPL) is present in large processes (arrows) morphologically similar to horizontal cell axons. Mel1b immunoreactivity (green) in the OPL is present in a distinct set of slender processes that form periodic clusters (arrowheads). Mel1b labeling is also present in the cell bodies of bipolar and amacrine cells (BC and AC, respectively) in the inner nuclear layer (INL). Mel1b labeling is also present at the level of the photoreceptor inner segments (PH). Mel1a and Mel1b receptors show differential distribution in the inner plexiform layer (IPL). Mel1b receptors are diffusely distributed throughout the IPL, but Mel1a receptors are found in discrete puncta (small arrows). The box in (A) indicates the area that is shown at higher magnification in (B–D). The confocal image is comprised of 16 optical slices of ≈400 nm. B–D: Examination of single confocal optical planes confirms that Mel1a and Mel1b localize to distinct processes in the OPL. Single optical slice of ≈400 nm is shown in B–D. B: Mel1b is present in slender processes arising from bipolar cells (BC) that form clusters in the distal OPL (large arrowhead). Mel1b labeling also is present in bipolar cell axons (arrow). C: Mel1a labeling is present in relatively large processes (small arrowheads). D: Overlay of panels B,C. Nuclei are counterstained with DAPI (blue) in all panels. Scale bars = 10 μm in all panels. |