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Figure 3. Summary of effects of GATA4 and GATA5 MOs in Xenopus embryos. A: Examples of phenotypic classes caused by GATA5 and GATA4 MOs. Ventral views of embryos injected with G5UTR MO or G4 MO are shown (50 ng/embryo). Heart and liver precursors have been revealed by MLC2 (BCIP) and FOR1 (BM purple) probes, respectively. FOR1 was developed first. Ventral views are shown, with anterior at the top. B, C: Summary of frequencies with which the heart and liver phenotypes were observed for GATA5 MOs (B) and for GATA4 MOs (C). The doses of splicing MOs are indicated and for other MOs are as in Figs. 1 and 4. |
