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Fig. 8. Morpholino MoXp54 impairs expression pattern of proneural genes. Embryos are injected at the two-cell stage into one blastomere with either 4 ng of a control morpholino (MoC), 4 ng of Xp54nrb morpholino (MoXp54) or co-injected with 4 ng of MoXp54 and 2 ng of human p54nrb mRNA. Embryos are raised to stage 14 or to stage 17, fixed and analysed by whole mount in situ hybridization for the expression of POU2 (A-D,M), Zic3 (E-H,N) and Sox2 (I-L,O). The side of injection is visualized by the b-galactosidase enzymatic reaction with Red-Gal substrate. Injected side is to the right.The embryos are shown in anterior views, dorsal is up.The patterns observed are representative of the embryos analysed. |